Mining for Restriction Endonucleases in Nicaragua

Autores/as

  • Suyén E. Espinoza-Miranda University of Central America
  • Julio A. Gómez-Rodríguez University of Central America
  • Jorge Huete-Pérez University of Central America

DOI:

https://doi.org/10.5377/encuentro.v0i93.910

Palabras clave:

bioprospecting, database, endonucleases, restriction enzyme

Resumen

The Molecular Biology Center at the University of Central America in Nicaragua (CBM-UCA) was founded in 1999 to strengthen biotechnology research capacity and education in Nicaragua and the Central American region. One of the first projects launched by the CBM-UCA was bio-prospecting for key industrial enzymes. This ongoing study seeks to discover and characterize restriction enzymes (RE) in bacteria, and to create a database of microorganisms isolated and identified by 16S rDNA sequencing methodology. In this paper we highlight the importance of studying the extreme environmental conditions for building knowledge of Nicaraguan biodiversity through modern molecular biology techniques such as metagenomics. The isolation of prototype enzymes such as EcoRV and ClaI is presented as an update and extension of previously undertaken work.

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Biografía del autor/a

Suyén E. Espinoza-Miranda, University of Central America

Molecular Biology Center

Julio A. Gómez-Rodríguez, University of Central America

Molecular Biology Center

Jorge Huete-Pérez, University of Central America

Molecular Biology Center

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Publicado

2012-12-10

Cómo citar

Espinoza-Miranda, S. E., Gómez-Rodríguez, J. A., & Huete-Pérez, J. (2012). Mining for Restriction Endonucleases in Nicaragua. Encuentro, (93), 49–62. https://doi.org/10.5377/encuentro.v0i93.910

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