USE OF ENDOPHYTIC INSULATION OF Trichoderma spp., FOR BIOCONTROL OF PANAMA DISEASE (Fusarium oxysporum f. Sp. Cubense) RACE I, IN VITRO PLANT

Authors

  • Álvaro José Caballero Hernández M.Sc. Agricultura Ecológica, Departamento de Agroecología Tropical. Facultad de Ciencia y Tecnología. Universidad Nacional Autónoma de Nicaragua-León
  • Luis Ernesto Pocasangre Enamorado Ph.D. Patología de Plantas, Universidad EARTH
  • Fernando Casanoves Ph.D. Ciencias Estadísticas, Unidad de Biometría, CATIE
  • Jacques Avelino Ph.D. Fitopatología, CATIE-CIRADH, Turrialba
  • Ana Cecilia Tapia Fernández M.Sc. Fitopatología, Sede de Atlántico, UCR, Turrialba
  • Juan Luis Ortiz M.Sc. Recursos Filogenéticos y Biotecnología, Laboratorio de Biotecnología, CATIE, Turrialba

DOI:

https://doi.org/10.5377/calera.v13i20.1620

Keywords:

Fusarium oxysporum f. sp. cubense, Panama disease, banana, Gros Michel (AAA), endophytic fungus, biological control, Trichoderma spp.

Abstract

The objective of this research was to select isolates of endophytic fungi of Trichoderma spp., for the biocontrol of Fusarium oxysporum f. sp. cubense race 1. We evaluated the three most pathogenic isolates FOC2, FOC4, FOC8 cryobank obtained from the Laboratory of Plant Pathology and Nematology at CATIE, in a test of antibiosis and then proceeded to test twenty biocontrol isolates of Trichoderma spp., and two isolates FOC4 and FOC2 in vitroplants of banana Gros Michel (AAA) under greenhouse conditions. Through the Coculture technique twenty endophytic fungi inhibited radial growth of Foc up to 53, 46% in comparison with reference control. In the bioassay of Biocontrol, most treatments showed the first symptoms after the third week. Protected plants with endophytic fungi delayed the appereance of the disease until the fourth week. The results showed that endophytic fungi showed a small percentage of incidence being the TJ5 who excelled with the lowest percentage of 37,5%, without showing any symptoms absolute control. Also TC9 endophytic fungi, TP3 and TCL1 reduced from 92% to 90% external symptoms, compared to reference control. Corm internal symptoms were reduced to74% by the endophytic fungus TC9. Additionally it was found that protected plants endophytic fungi in promoting growth in the variables of foliage weight and root weight.

DOI: http://dx.doi.org/10.5377/calera.v13i20.1620

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Published

2014-11-30

How to Cite

Hernández, Álvaro J. C., Pocasangre Enamorado, L. E., Casanoves, F., Avelino, J., Tapia Fernández, A. C., & Luis Ortiz, J. (2014). USE OF ENDOPHYTIC INSULATION OF Trichoderma spp., FOR BIOCONTROL OF PANAMA DISEASE (Fusarium oxysporum f. Sp. Cubense) RACE I, IN VITRO PLANT. La Calera, 13(20), 16–23. https://doi.org/10.5377/calera.v13i20.1620

Issue

Section

Plant Protection