Immunoreactivityof the protein p53 in premalignant and malignant cervical lesions

Abstract

Objective: To determine the expression p53 in malign and pre-malignlesions of the cervix in the patients of the Consulta Externa of the Service of Gynecology of the Hospital Materno Infantil during the period of July 2008 to December 2009.

Material and method: A study of cross-sectional descriptive type. Studying a sample of 90 patients of a Universe of 209. The patients were characterized according to their risk factors, in addition biopsy by colposcopy and/or conizacion to the patients with clinical suspicion of malign or pre-malign injury of uterine cervix were made. The biopsies were including in paraffin blocks for the staining with Hematoxilina - Eosin and posterior the recovery of the antigen p53, using a policlonal antibody by means of the inmunoperoxidase technique of, for its later statistical analysis.

Results: The mean age was of 40,9 years (rank 18-105 years). The average schooling was of 6,8 years, with rank of 1 to 15 years. 50% (45) of the patients referred to have had single a sexual pair. The 92,2% (83) of the biopsies were in punch and 7,8% (7) were cones. Of the biopsy results 10% (9 cases) were invasive carcinoma of squamous cells, 23,3% (21 cases) High degree intraepithelial lesions - CIN III, CIN II 23,3% (14 cases), 33,3% (30 cases) where CIN I and 16 cases (17,8%) corresponded to other injuries where chronic endocervicitis, endocervical, polyp by atypical reparative changes were included. The identification of the protein p53, was made in 73 cases previously classified as malign or pre-malign lesions of the cervix, obtaining the following results; 6 of 9 cases of of carcinomas of squamous cells (66,67%) presented/displayed nuclear positividad, NIC III 12/21 positive cases (57,14%), NIC II 3/14 positive cases (21,42%), NIC I 12/30 positive cases (40%).

DOI: http://dx.doi.org/10.5377/rct.v0i7.1804

Revista Ciencia y Tecnología No. 7, diciembre 2010: 63-73