Viability of spores of Aspergillus awamori immobilized in cryogels

Authors

DOI:

https://doi.org/10.5377/rtu.v10i29.12739

Keywords:

Immobilization by entrapment, polivinyl alcohol

Abstract

The work consisted of immobilizing Aspergillus awamori spores in polyvinil alcohol (PVA) at different concentrations 10, 12 y 15% (w/v).  The viability of the immobilized spores was compared to the free spores, by the conventional plate counting method.  The imobilization technique was by entrapment, it consisted of a suspension of spores in the polymer and then it was frozen at -18 ºC for 24 hours.  Viability was similar in immobilized and frees pores, this indicates that there are probably no toxic effects of the polymer and that exposure to low temperatures does not damage the structure of the spores.  Spores leakage was different in each case of immobilization at 24 hours, the least leakage was with spores at 15% and the highest at 12%.  At 96 hours, the lowest was with spores immobilized at 10%, followed by 15% and the highest spores immobilized at 12%, thus remaining until 120 hours.

Downloads

Download data is not yet available.
Abstract
384
PDF (Español (España)) 206
HTML (Español (España)) 120
VISOR (Español (España)) 0
PDF 59
HTML 126

References

Efremenko, E. N., Spiricheva, O. V., Veremeenko, D. V., Baibak, A. V., & Lozinsky, V. I. (2006). L(+)-lactic acid production using poly(vinyl alcohol)-cryogel-entrapped Rhizopus oryzae fungal cells. Journal of Chemical Technology and Biotechnology, 81(4), 519–522. https://doi.org/10.1002/jctb.1524

Efremenko, E., Spiricheva, O., Varfolomeyev, S., & Lozinsky, V. (2006). Rhizopus oryzae fungus cells producing L(+)-lactic acid: Kinetic and metabolic parameters of free and PVA-cryogel-entrapped mycelium. Applied Microbiology and Biotechnology, 72(3), 480–485. https://doi.org/10.1007/s00253-005-0297-y

El-Mansi, E. M. T., Stephanopoulos, G., & Carlson, R. P. (2011). Flux control analysis and stoichiometric network modeling: Basic principles and industrial applications. Fermentation Microbiology and Biotechnology, Third Edition, December 2011, 165–200. https://doi.org/10.1201/b11490-13

Lozinsky, V. I., & Plieva, F. M. (1998). Poly(vinyl alcohol) cryogels employed as matrices for cell immobilization. 3. Overview of recent research and developments. Enzyme and Microbial Technology, 23(3–4), 227–242. https://doi.org/10.1016/S0141-0229(98)00036-2

Plieva, F. M., Galaev, I. Y., Noppe, W., & Mattiasson, B. (2008). Cryogel applications in microbiology. Trends in Microbiology, 16(11), 543–551. https://doi.org/10.1016/j.tim.2008.08.005

Willaert, R. G., & Baron, G. V. (1996). Gel entrapment and micro-encapsulation: Methods, applications and engineering principles. Reviews in Chemical Engineering, 12(1–2), 160–205. https://doi.org/10.1515/revce.1996.12.1-2.1

Published

2021-11-01

How to Cite

Plata Oviedo, M., Pérez, Z., Lacayo Romero, M., & Gazo, G. (2021). Viability of spores of Aspergillus awamori immobilized in cryogels. Torreon Universitario Magazine, 10(29), 129–135. https://doi.org/10.5377/rtu.v10i29.12739

Issue

Section

Sciences

Most read articles by the same author(s)